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Dunn Labortechnik GmbH
Reliable Sample Preparation for Single-Cell Genomics with the Singulator™ from S2 Genomics
Whether samples are analyzed by scRNA-seq, ATAC-seq, snRNA-Seq, CITE-Seq or other ‘omics applications, one of the most important factors for Single-cell genomics data quality and thereby key to meaningful data, is sample quality. Results should be reproducible, and cells and nuclei isolated as quickly and efficient as possible to ensure high viability and yield. Finding the ideal protocol, as well as skilful hands, is a difficult task and can take a lot of time and effort.
The Singulator™ system simplifies tissue dissociations into single-cell suspensions and nuclei suspensions to the press of a button, reducing variability due to handling, and shortening isolation times to 6 – 80 minutes. Fresh and frozen tissues, as well as OCT and FFPE-imbedded samples can be processed with the Singulator™ system.
Samples as low as 2 mg of tissue are transferred into specially designed cartridges, where the tissue is automatically mechanically disrupted, digested or lysed and filtered to remove large amounts of debris. Afterwards, the single-cell/single nuclei suspension is removed and can be used for downstream processing.
To learn more about the device, schedule your online demo now or visit us at the upcoming EMBL | EMBO Symposium from 10th to 13th June in Heidelberg.
BelloCell® - The high yield "Tide-Motion" Bioreactor System for adherent cells from Esco Bioengineering
Dunn Labortechnik presents the BelloCell® bioreactor system from Esco Bioengineering for high density cultivation of adherent growing cells in both serum-containing and serum-free culture medium. The BelloCell® system is widely used by researchers and bioprocess specialists for a large variety of animal, mammalian and insect cell culture applications in CO2 incubator. Using the optional BelloFeeder® pump module and media bottles as external reservoirs, the BelloCell® bioreactor can easily be converted into a continuous (perfusion) reactor system (see pictures above showing a batch type and a continuous BelloCell® system).
The large specific surface area of up to 2.730 cm2 per gram of the used standard BioNOC II® macrocarriers and flexible design of the BelloCell® reactor bottles allow precisely controlled, alternating exposure of microcarriers to air/atmosphere and culture medium (tidal flow principle, see above) facilitating optimal supply of nutrients and gases and at the same time applying very low shear stress and preventing foam formation. Applications of BioNOC II® macrocarriers range from protein expression and production of non-secreted proteins to the production of (stem) cells, secreted proteins, viruses and monoclonal antibodies and these carriers have been successfully used with a large variety of adherent cell lines.
Available from Dunn Labortechnik are now the new BioMESH® macrocarriers. BioMESH® are the next generation grid-like PP/PET macroporous carriers (see below) with low-lint and low particulate contamination.
BioMESH® carriers are specifically designed for high density cell harvesting and recommended for stem cell expansion and cell therapy applications. Applications also include cultivated meat, production of EVs/exosomes and secreted bioproducts like viruses and proteins. Examples of cultivated cells are multipotent/pluripotent stem cells, epithelial cells, chondrocytes, fibroblasts and fibroblast-like cells (e.g. skin, cardiac fibroblasts).