Newsletter
ELISA Kits and Components
Featured Kits, New Online Resources and Useful Tools
Innovative Research provides two new video resources on their website where they answer frequently asked questions about ELISA kits and explain the difference between active and total ELISA kits.
Highlights from their extensive portfolio are their numerous Coagulation Factor ELISA Kits to quantify clotting dynamics as well as their IgG Subclass ELISA Kits, ideal for profiling patient-specific immune responses.
ICL Inc. offers 14 Host Cell Proteins (HCP) ELISA kits for targeted and highly specific detection of CHO cells including PLBL2, a common CHO-derived impurity linked to immunogenicity and drug degradation or other key CHO-associated proteins like CTSA, LPLA2, Clusterin, and MMP-19 which are often missed by generic assays.
Detecting and removing HCP is essential when manufacturing biologic drugs to ensure safety and efficacy. Nowadays, generic host cell protein assays are often not sufficient anymore. Instead, protein specific ELISA kits in combination with orthogonal testing methods are preferred.
MOSS Inc. specialized in producing high-quality, liquid-stable substrates. Depending on your ELISA needs, you can choose from different chemiluminescent or chromogenic, soluble substrates for Horseradish peroxidase (HRP) and Alkaline phosphatase (AP), for example Chemi-HRP, Chemi-AP, TMB, ABTS or p-NPP.
For TMB, several different compositions are available with varying reactivity and sensitivity. To find the optimal TMB substrate for your assay, you can now order the new TMB evaluation pack TMB-SK. This pack contains all available soluble TMB substrates as well as their diluents. More information can be found in our brochure.
New High-bind plates from Porvair Sciences
The new Krystal™ Signum High Bind clear 96-well flat bottomed plates are designed to bind proteins, peptides, antibodies or other biomolecules onto the plate surface. These characteristics make them valuable for a wide range of applications such as ELISA, antibody screening/production, protein assays, peptide or hapten binding and general biomolecular immobilization. The two key characteristics are the high activation level and good clarity of the plate.
The new plates are made from high-grade polystyrene having a low background ideal for ELISA and fluorescence. The combination of a hydrophilic and hydrophobic surface allows binding a wide range of different molecules such as proteins, peptides and antibodies. All plates are tested for uniformity, stability and reproducibility and come in a standard SLAS/ANSI-format.
You are not convinced yet? Ask for a sample or use our launch offer to get a package of these plates with nearly 50 % off list price.
Neu Microlute® von Porvair Sciences
SPE mit höchster Reproduzierbarkeit
Microlute® CSi und CP führen SPE auf ein neues Level durch die Erhöhung der Reproduzierbarkeit der Extraktion von Analyten und Ausbeute von biologischen, ökologischen und chemischen Proben.
Die neue Microlute® Hybridtechnologie besteht aus einem festen, verzweigten Netzwerk aus gleichmäßig verteilten Poren, welche mit immobilisiertem Chromatographiemedium beladen sind. Microlute® CP nutzt dabei Polymere und Microlute® CSi Silica.
Diese Struktur sorgt für einen gleichmäßigen Durchfluss der Proben und maximiert die Interaktionen zwischen den Analyten und der festen Phase, was zu einer hohen Reproduzierbarkeit der Methode in den Bereichen Durchführung, Sauberkeit und Sensitivität führt.
Die Microlute® Produkte gibt es als 96-Well Platten mit einem Füllvolumen von 2 oder 10 mg. Verfügbare Produkte sind C18 und HLB zur Retention von neutralen Analyten über hydrophobe Interaktionen, SCX bestehend aus einer Schwefelsäuregruppe und polymeren Basen mit einem pKa kleiner 1 für die Bindung starker Kationen und SAX bestehend aus einer quaternären Ammoniumchemie und polymeren Basen mit einem pKa größer 18 zur Bindung von starken Anionen.
Diese weitreichende Chemie macht die Microlute® Produkte ideal für die Anwendung in der Medikamentenentwicklung, klinischen und toxikologischen Studien sowie forensischen Laboratorien.
Profitieren auch Sie von der Reproduzierbarkeit der Microlute® Produkte und fragen noch heute ihr Angebot an.
Kontakt:
Dr. Kevin Denkmann • Geschäftsführer
Dunn Labortechnik GmbH • Thelenberg 6 • 53567 Asbach
Tel. +49 (0) 2683 4 30 94 • E-Mail: info@dunnlab.de • Internet: www.dunnlab.de
Dunn Labortechnik GmbH
New in our portfolio: ECIS® from Applied BioPhysics
We are happy to announce our new partnership with the US-based company Applied BioPhysics. As new exclusive distributor for the DACH-region, Belgium and the Netherlands, we will be supporting existing customers and take care of new enquiries.
Applied BioPhysics, in the market for over 3 decades, is known for Electric Cell-substrate Impedance Sensing (ECIS®) for non-invasive and label-free monitoring of cells. They offer the system Z-Theta which measures impedance and capacities via gold electrodes in cell cultures and the TEERZ measuring TEER-values with dripping pin electrodes in standard filter insert plates.
These measurements are useful for a wide range of possible applications such as barrier function, cell migration, cell proliferation, cytotoxicity, wound healing, drug research, and many more.
A specific tool of ECIS® for wound healing is integrated in the user-friendly software of the device. High currents for a defined period of time are applied to kill the cells over the electrode. If these voltages are used for a very short period even electroporation methods are possible.
Applied BioPhysics offers a wide range of arrays for their devices also enabling special assays including experiments under flow.
Flexcell® Cell Stretching Bioreactors for Life Science Research
(Tension, Compression, Fluid Shear Stress)
Particularly designed for the in vitro analysis of biomechanical applications, Flexcell´s innovative systems enable the user to induce and observe the results of cyclic or static stress on 2D/3D cell cultures and tissues.
For tension applications, Flexcell offers the FX-6000 Tension System which functions with both regulated vacuum pressure and positive air pressure to effectively move the membranes of flexible-bottomed culture plates.
Flexcell’s Compression System regulates positive air pressure to compress tissue samples or 3D cell cultures in vitro. The FX-5000™ Compression System compresses samples between a piston and stationary platen using special BioPress™ culture plates. It can load up to 14 lbs. of applied force.
The user-friendly “Streamer” Shear Stress Device and the optionally available accessories “FlexFlow” and “Osci-Flow” allow the investigation of shear stress effects on 2D cell cultures.
Furthermore, Flexcell offers various Microscope Devices to be used with the Tension and Compression Systems.
All Flexcell products can be purchased through the exclusive distributor for Europe, Dunn Labortechnik, in Germany.
