Bellco Glass Inc. – Consumables

Bellco Glass Inc. – Consumables

Excerpt from our Bellco’s consumables portfolio.

  • Anaerobic Tubes and Culture Bottles
  • Caps and Closures (KAP-UTS™, stainless steel, silicone sponge, screw caps)
  • Cloning Cylinders
  • Cover Slips (rectangular, square, round, with Photo-eched or without)
  • Flasks and Bottles  (Biometer-, DeLong®-, Erlenmeyer-, Fernbach-, Nephelo-, Roux-, Shake-, Snap-Seal-, Trypsinizing-, Volumetric)
  • Filling Bell, aseptic
  • Homogenizers, BioVortexers, Tissue Grinders, Cellector™ Tissue Sieve
  • Microscope Slides
  • Pipette Container
  • Racks for tubes
  • Roller Bottles
  • Serological Glass Pipettes
  • Spinner Flasks and Bioreactor Vessels and Accessories
  • Sykes-Moore Chamber
  • Tubes
  Please note the entire Bellco product range (PDF)

Anaerobic Tubes and Culture Bottles

Hungate culture tubes

  • Gas impermeable tubes for anaerobic culture conditions
  • Sampling and additon of nutrients via syringes
  • Consists of:
    • Culture tubes made of glass with round base and screw thread
    • Gas-impermeable, black butyl rubber stopper
    • Screw cap

 

Anaerobic culture tubes with aluminum caps (Balch type)

  • Gas-impermeable tubes made of borosilicate glass for anaerobic culture conditions
  • Particularly suitable for studies on bacteria that produce methane gas
  • Gas-impermeable, blue septa made of butyl rubber

 

Anaerobic culture bottles

  • Borosilicate glass type 1, class A
  • Innovative 45 mm PP screw cap (GL45) with silicone O-ring and blue septum plug made of butyl rubber, designed to maintain anaerobic conditions
  • Sizes: 100, 250, 500 and 1000 ml

 

DeLong® culture bottles

  • Ideal for growing, storing and shaking aerobic and anaerobic cultures
  • Borosilicate glass type 1, class A
  • Graduation with approximate volume
  • Available with stainless steel caps or KAP-UTS ™ or without caps

 

Crimping and unlocking pliers for anaerobic culture tubes and bottles

further information about the Bellco anaerobic bottles (PDF)

Cover Slips

Square and rectangular cover slips

  •  Manufactured from Corning 0211 Glass
  • Glass thickness: 0.13 mm – 0.16 mm
  • Different sizes available

 

Round cover slips

  • Manufactured from German Glass D 263M
  • Glass thickness: 0.13 mm – 0.16 mm
  • Diameter: 5 mm to 25 mm
  • High chemical resistance
  • Ideal for neuronal tissue cultures and less adherent cells
  • Suitable for fluorescence and confocal microscopy

 

further information about the Bellco Cover Slips (PDF)

Photo-etched Cover Slips

Coding facilitates micromanipulation of cells or analysis of chromosome spreads

Round cover slips

  • Manufactured from German Glass D 263M
  • Coded area: 0.6 mm x 0.6 mm
  • 100 (ø: 12 mm) or 200 (ø: 25 mm) alphanumeric coded squares
  • Glass thickness: 0.13 mm – 0.16 mm

 

Square cover slips

  • Manufactured from German Glass D 263M
  • Coded area: 0.6 mm x 0.6 mm
  • 520 alphanumeric coded squares
  • Glass thickness: 0.19 mm – 0.25 mm

 

further information about the Bellco Cover Slips (PDF)

Erlenmeyer and Shake Flasks

  • Manufactured from type 1 class A Borosilicate glass
  • With DeLong® neck, break resistant lip or screw cap
  • Narrow or wide mouth
  • Different versions:
    • Without or with bottom and/or side baffles
    • Extra deep side baffles
    • 4 extra deep bottom baffles and constriction
  • GL45 solid or vented screw caps
  • Capacity range from 25 ml to 6 l

 

further information on the Bellco Erlenmeyer Flasks (PDF)

Fernbach Flasks

  • Ideal for bacterial cultures with high oxygen requirements
  • Flask made of type 1 borosilicate glass, class A
  • DeLong® neck with break-resistant lip, GL45 solid or membrane screw cap for fast pressure compensation (closed lid or ventilation lid)
  • Without or with 3 bottom and/or 3 side baffles
  • Capacity: 1800 ml or 2800 ml

 

See also “Flasks and Bottles”

further information on the Bellco Fernbach Flasks (PDF)

Flasks and Bottles from Borosilicate glass, different styles

Biometer flasks

  • To measure microbial CO2 production
  • Delivery funnel to the flask for addition of e.g. acids and bases
  • Second chamber to collect and harvest gas
  • Made from Borosilicate glass type 1, class A
  • White or brown glass for lightsensitive materials
  • Autoclaveable

 

Hybridization Bottles Autoblot®

  • Designed for DNA/RNA blotting procedures
  • Type 1, class A Borosilicate glass
  • Includes red PBT high temperature cap

 

Nephelo Culture Flasks

  • DeLong® neck with stainless steal closures or phenolic rubber lined screw caps
  • With or without cleanout port
  • Also available with baffles for increased oxygenation

 

Roux flasks

  • With screw thread or DeLong® neck
  • Volume: 1200 ml

 

Trypsinizing flasks

  • With 3 deep side baffles for optimal aeration
  • With break-resistant lip or screw thread
  • Without or with sidearm and constriction below sidearm for retention of unseparated material
  • Borosilicate glass type 1, class A

 

Volumetric flasks

  • Clear glass or amber stained glass for light sensitive materials
  • Meet ASTM E288 specifications
  • Permanent graduation line and marking area

 

See also: “Erlenmeyer and Shake Flasks”

See also: “Fernbach Flasks”

further information on the Bellco Flasks & Bottles (PDF)

Racks for test and culture

Leighton culture tube racks

  • For 36 Leighton tubes
  • 2 types available for tube lengths 83 – 93 mm and 125 – 150 mm

 

Roller culture tube rack for rotating culture tubes (roller tubes) / Hungate culture tubes

  • Made of stainless steel
  • For 64 positions
  • Tube Ø max 16 mm

 

Test and culture tube racks made of stainless steel

  • Rustproof and resistant in a water bath or in CO2 atmosphere
  • Different sizes available for all common tube shapes

 

further information on page 27 of the Bellco catalogue (PDF)

Filling Bell

  • Aseptic filling bell from Borosilicate glass Type 1, class A
  • Attaches to reservoir with rubber tubing
  • Receiving vessel is placed under the bell
  • Different sizes available for use with tubes or bottles
  • Autoclavable

 

further information on page 16 of the Bellco product range (PDF)

Homogenizers, BioVortexers, Tissue Grinders

A wide range of manual and semi-automatic homogenizers is available for a large number of applications in the field of disruption of tissues, cells and cell organelles.

Manually operated homogenizers (Glass)

  • Dounce homogenizers
    • For cell disruptions
    • For extraction of cell nucleuses

 

  • Conical shaped (Glass)
    • Ideal for disruption of heart, skeleton-muscle and lung tissue

 

  • Potter-Elvehjem homogenizer
    • Entirely made of glass or with a pestle consisting of a stainless steel shaft and a PTFE-head
    • For disruption of tougher tissues, connective tissues (glass pestle) and soft tissues like liver or brain (PTFE-head)

 

  • Tenbroeck homogenizer
    • With hollow glass pestle for cooling

 

  • Cellector® Tissue Sieve
    • For the preparation of single cell suspensions and for gel extraction of proteins
    • Entirely made of stainless steel
    • Two dish sizes: 85 ml and 130 ml
    • Available in mesh sizes from 25 Μm to 1910 Μm

 

Semi-automated homogenizers

  • BioVortexer
    • Batterie-powered homogenizer
    • Single use pestle for use with 0.5 ml  and 1.5 ml tubes

 

  • Overhead drives

 

further information on pages 25 -26 of the Bellco catalogue (PDF)

Cloning Cylinders

  • For the isolation of single cells or clones from mixed populations on solid culture media (culture dishes)
  • Four sizes available: 6, 8, 10 and 16 mm outer diameter
  • Made from Borosilicate glass (or on request or in stainless steel)
  • Suitable for heat sterilization and autoclaving

 

further information on page 16 of the Bellco Catalogue (PDF)

Microscope Slides

  • Pre-cleaned microscope slides, 25 x 75 mm, plain or with one/two frosted ends
  • Reich counting slides with three rings of 1 cm2 interior area, for cell counting and calculation of bacterial sample cell concentration
  • Cell counting slides for e.g. counting of somatic cells in cow’s milk. Spread 10 µl of sample onto each circle, dry, stain and count
  • Fluorescent antibody slide with black ceramic background and 8 rings (6 mm diameter) for indirect fluorescent antibody tests

 

further information about the Bellco Cover Slips (PDF)

further information on pages 17 -18 of the Bellco catalogue (PDF)

Pipette Containers

Pipette container from Borosilicate glass

  • Round bottom or flat bottom with silicone pad
  • PTFE cushion for round bottom containers available
  • 38 mm diameter
  • Different lengths available, also for „shortie“ pipettes
  • Autoclavable and dry heat sterilisable
  • Mounting plate to stabilize round containers

 

Pipette container from stainless steel

  • Rectangular construction for easy stacking and storage
  • Inside silicone pads at top and bottom
  • Different sizes available
  • Autoclavable and dry heat sterilisable up to 180 °C

 

further information on pages 30 – 31 of Bellco catalogue (PDF)

Tubes

Centrifuge Tube

  • Type 1, class A or B Borosilicate glass
  • With 38 mm rubber lined phenolic screw cap
  • Capacity: 590 ml or 780 ml
  • Fits into I.E.C. No. 353A centrifuge adapters

 

Culture Tubes

  • Type 1, class A or B Borosilicate glass
  • Plain top to be used with KAP-UTS™ or stainless steel closures
  • Different diameters and lengths
  • Round bottom

 

Gas Fermentation Tube

  • To be inverted in bottom of larger tubes for collection of fermentation gases
  • Standard flint glass 1.5 mm thick
  • Lenght 29 mm, Ø 8 mm

 

Leighton Tubes

  • Type 1, class A Borosilicate glass
  • Rectangular coverslips suitable for Leighton tubes available
  • Short neck for easy positioning of cover slips
  • Plain top or screw cap available
  • Fits into Leighton tube rack (also available)

 

further information on pages 27 – 28 of  Bellco catalogue (PDF)

Roller Bottles

  • Type 1, class A Borosilicate glass
  • Diameter 11 cm to 12 cm for use with roller apparatus
  • Different screw caps available:
    • 38 mm long skirted, black phenolic, rubber lined
    • 45 mm (GL45) PP caps
    • 51 mm flat, black phenolic, rubber lined
  • Different lengths available
  • Growth areas from 670 cm² to 1,585 cm²

 

further information on the Bellco Roller Bottles (PDF)

Serological Glass Pipettes

  • Type 1, class A Borosilicate glass
  • Various styles available
  • With or without cotton plugging top
  • Blow out, tempered tip
  • Calibrated to the tip
  • Available: 3 mm orifice for handling tissue homogenates and cell suspensions
  • “Shorties” for easy use under biological hoods
  • Plug your pipettes with the suitable Pipette Plugger

 

further information on page 30 of the Bellco catalogue (PDF)

Spinner Flasks and Bioreactor Vessels

Different types of spinner flasks and bioreactor vessels for various applications: suspension of bacteria, insect, mammalian or plant cells, as well as microcarrier culture. To be used with magnetic stirrers or overhead drives.

  • Many sizes available: from 25 ml to 36 l
  • Unjacketed and jacketed flasks
  • Central neck: 70 mm or 100 mm
  • Sidearm necks: GL32 or GL45
  • Made from type 1, class A Borosilicate glass

 

Spinner flask types available

  • Internal impeller assembly or height adjustable shaft
  • With different types of impeller blades or magnetic stir bar only
  • Spinner flasks with 2 or 4 sidearms
  • Flat bottom or dimpled bottom to prevent cell accumulation
  • MicroCarrier flask
  • Internal impeller flask
  • BELL-FLO™ flask with vertical baffles
  • Glass Ball flask for gentle agitation
  • Adjustable hanging bar flask
  • BioProbe™ flask with 4 side arms
  • Water-jacketed flasks

 

Bioreactor types available

  • 4-45™ bioreactors with 4 GL45 sidearms
  • Vertical Sidearm Bioreactor Vessels with 4 or 6 GL45 sidearms
  • Lid with stainless steel connections (different variants and number of access ports) available
  • Water-jacketed bioreactor vessels
  • High or low profile vessel

 

further information on the Bellco Spinner Flasks (PDF)

Spinner Flask and Bioreactor Accessories

  • Flasks Carriers
  • Sparger and sparger assemblies
  • Caps and impeller assemblies
  • Probes and sensors (pH, oxygen, temperature)
  • Shafts (for GL 45 caps)
  • BioSimplex ™, sterile
  • Various port assemblies
    • 2-Port assemblies
    • 3-Port assemblies with or without sparger
    • Thermowell assemblies
  • Suitable open top caps and solid caps (please see above „Caps and Closures“)

 

further information on the Bellco Spinner Flasks (PDF)

Sykes-Moore Chamber

  • For the study of cells by means of high magnification, phase optics, or time lapse techniques
  • Cover slips are separated by a nontoxic silicone gasket
  • The chamber has a 2.8 cm2 viewing area and holds approx. 650 to 750 Μl

 

further information on page 18 of the Bellco catalogue (PDF)

Caps and Closures

GL screw caps

  • Polypropylene, inner sealing lip, autoclavable up to 140 °C, different colours
  • PTFE/silicone liner,  32 mm and 45 mm
  • Screwcap for high temperatures, PTFE/silicone liner, -196 °C to +260 °C, clear, USP Class VI, EP and FDA compliant
  • Cap with Μm PTFE membrane, autoclavable
  • PP Open top polypropylene caps for use with PFTE/silicone septa or access port assemblies
  • Pouring rings for GL32 and GL45 available

 

KAP-UTS™ disposable closures

  • For culture tubes and flasks with DeLong® style necks
  • Manufactured from nontoxic polypropylene, autoclavable
  • Seven colours and six sizes from 13 mm to 38 mm inner diameter
  • Same evaporation and sterility characteristics as stainless steel closures
  • Moulded pressure fins for firm grip
  • Autoclavable up to 140 °C

 

Phenolic screw caps (bakelite) with liner

  • Screw caps with white rubber or PTFE bonded liner
  • Long cap design reduces risk of cross contamination
  • Autoclavable (steam sterilization)

 

Silicone sponge closures

  • Sealed-in silicone sponge filter is ideal for aerobic  cultures
  • Flanged to fit snugly over outside of vessel necks
  • Withstand dry heat sterilization up  to 180 °C

 

Silicone sponge plugs

  • Tapered silicone sponge with smooth exterior for  tight seal
  • Extra long for easy insertion and removal

 

Stainless steel closure

  • For culture tubes and flasks with DeLong® style  necks
  • Manufactured from 305 stainless steel
  • With finger grips for firm fit
  • Withstand dry heat sterilization and autoclaving

 

further information on the Bellco caps (PDF)

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