Karriere im Labor und Vertrieb bei Dunn Labortechnik
Karriere im Labor und Vertrieb bei Dunn Labortechnik

Porvair Sciences Chromatrap

Porvair Sciences Chromatrap

  • Antibodies and Primers for ChIP assays
  • ChIP-Seq Kits
  • ChIP-Seq HT 96-well Plates
  • Chromatrap DNA Extraction
  • Chromatrap Homogenizer Spin Columns
  • DNA-Kits for Extraction, Purification, Concentration and Size Selection
  • gDNA Removal prior to RNA Extraction
  • Specialised ChIP Kits – Native
  • Specialised ChIP Kits – FFPE
  • Specialised ChIP Kits – Drosophila
  • Shearing Kits
  • UniqSeq Kits
 

Solid-State Chromatin Immunoprecipitation

Chromatin immunoprecipitation (ChIP) with the patented bead-free technology from Chromatrap® provided from Porvair Sciences utilizes filters with an inert porous polymer matrix coupled with Protein-A or Protein-G on the surface. This increased surface area maximizes immunocapture of antibody-chromatin complexes from your samples.   Features:
  • Easy to use and simple spin column protocol with less manual handling
  • Highest sensitivity and binding capacity through optimally oriented Protein-A/G arrangement across filter (see Figure)
  • No sample loss as antibody-chromatin complexes are captured and retained on filter
  • Lowest background and exceptional signal-to-noise ratio compared to other ChIP kits
  • Lowest amount input material, highest DNA yield
  • ChIP from as low as 1000 cells, 50 ng chromatin or 5 mg tissue
  • Availabe as Protein-A/G spin columns or 96 well filter plates
  • Chromatin-to-Sequencing ready in under 5 hours
  • Applications: Next Generation Sequencing, PCR, RT-PCR, Mass Spectrometry 
Mehr erfahren
20210126T123414-RNAExtractionKit.430x430-2880w
ChromatrapTechnologyIllustrationTop-Final-1920w-1-1024x333
AntibodiesandPrimersforChIPassays-2880w
DNA-Extraction-Kit-2880w

Ansprechpartner

Dr. Kevin Denkmann

Kontaktieren Sie uns
zurück zur Übersicht

Ähnliche Produkte

Antibodies and Primers for ChIP assays

  • Use of high-quality, specific ChIP validated antibody is essential for the success of a ChIP assay
  • Positive and negative control targets
  • Can be supplied with primer sets for extra validation
  • Reduce time spent searching databases and designing primers
  • Confidence in correct targets for antibodies

 

ChIP-Seq Kits

  • Most sensitive kit at low cell numbers
  • Can be used with:
    • Cell lines, primary cells, and difficult cell types and tissues
    • Low abundant targets
    • Library preparation kits
    • All sequencing platforms (e.g. Inc Illumina, Ion torrent, Roche)

 

further information on Chromatrap products (PDF)

ChIP-Seq HT 96-well Plates

  • Perform up to 96 reactions simultaneously in 1 day
  • Pair with automated liquid handling for even higher throughput
  • Can be used for:
    • Antibody validation
    • Multiple antibodies
    • Multiple samples
    • Multiple cell types
    • Multiple drug compounds/inhibitors

 

further information on Chromatrap products (PDF)

UniqSeq Kits

  • Complete solution for ChIP and library preparation for next generation sequencing
  • Compatible with all Illumina sequencers
  • Low levels of duplication (< 2 %)
  • High (98 %) uniquely mapped reads
  • One tube reaction – End repair, A-tail & ligation in one step
  • Bead free size selection
  • Low input (500 pg DNA)

 

further information on Chromatrap products (PDF)

Specialised ChIP Kits – Native

  • Only commercial method to study chromatin in its ‘native’ state
  • No chemical fixation required
  • Cells are in more natural ‘native’ state
  • Can be used for:
    • Non-fixed samples
    • Histone modifications
    • Transcription factors tightly associated with DNA

 

further information on Chromatrap products (PDF)

Specialised ChIP Kits – FFPE

  • For use with formalin fixed paraffin embedded (FFPE) tissue
  • Can be used for:
    • Tissue samples
    • Biopsies
    • Difficult-to-lyse samples
    • Range of different FFPE tissue (human & animal)

 

further information on Chromatrap products (PDF)

Specialised ChIP Kits – Drosophila

  • Only commercial method to study Drosophila (fruit fly) chromatin
  • Focus on Drosophila community of researchers and neuroscientists
  • Uses as little as 5 flies or 500 ng chromatin
  • Can be used for:
    • Whole body samples
    • Larvae samples
    • Pupal samples
    • Embryonic samples
    • Histone modifications
    • Transcription factors

 

further information on Chromatrap products (PDF)

Shearing Kits

For optimisation of shearing conditions
 
Sonication
  • Simple and effective method for preparation of high-quality chromatin
  • Can be used for most cell types and tissues
  • Useful for difficult to lyse samples

 

Enzymatic
  • Suitable for native chromatin preparation
  • Effective alternative to sonication as no expensive equipment is required
  • Milder treatment, can be less disruptive to the epitopes

 

further information on Chromatrap products (PDF)

gDNA Removal prior to RNA Extraction

The new Chromatrap® genomic DNA (gDNA) Removal Columns can be used to eliminate gDNA along with RNA extraction recommended with Chromatrap® RNA Extraction kit. This procedure ensures maximum purity and yield of pure RNA from a wide range of samples in a fast and cost-effective way.

Features of Chromatrap® gDNA Removal Columns:

  • Enzyme-free gDNA removal from cell lysates within 30 seconds / sample
  • Load and spin method for gDNA-free, total RNA
  • Simple, one-step centrifugation method
  • Highly specific gDNA removal with no loss of precious RNA
  • Ideal for DNA-sensitive applications
  • Can be used as an upstream gDNA removal kit prior to RNA extraction

 

Features of Chromatrap® RNA Extraction kit:

  • Reproducible and fast extraction of high-quality total RNA
  • Compatible with a wide range of samples including buffy coat, buccal swabs, and gram-negative bacteria
  • Process up to 107 cultured animal cells, 50 mg animal tissue, 0.5 ml blood, 250 mg plant material, 5 x 107 yeasts or 5 x 108 bacteria
  • High yield extraction of up to 1000 Μg pure RNA
  • Application-ready total RNA for qPCR, Microarray or other in vitro applications

 

further information on Chromatrap products (PDF)

Chromatrap® DNA Extraction

  • Used to extract DNA from cells, tissues and blood samples
  • Allows highest amount of DNA recovery from starting sample (DNA recovery up to 98 %)
  • Purification of up to 50 Μg DNA
  • Samples are free from contaminants such as salts, inhibitors or enzymes
  • For applications such as PCR, ChIP, Southern blotting, DNA methylation studies, Molecular cloning and Sequencing
  • Phenol- and chloroform-free

 

further information on Chromatrap products (PDF)

DNA kits to extract, purify, concentrate and size select

  • Simple and fast procedure
  • In 96-well format for high-throughput
  • DNA Extraction: high quality DNA from up to 25 mg tissue or 5 million cells in 200 Μl
  • DNA Purification: removal of undesired impurities from up to 50 Μg DNA
  • DNA Purify & Concentrate: Purify and concentrate up to  10 Μg DNA in as little as 5 to 10 Μl
  • Size Selection: Bead-free exclusion of DNA fragments  < 200 bp

 

Chromatrap® Homogeniser Spin Columns

Quickly and efficiently homogenise cells and tissues lysates in a single step. Contained within a spin column, the unique proprietary bio-shredding Vyon® polymer reduces lysate viscosity and captures insoluble debris by centrifugation.
  • Perfect alternative to syringe & needle homogenisation
  • Quick, clean and efficient homogenisation
  • Reduces viscosity of lysate
  • Ideal sample preparation for nucleic acid miniprep and midiprep
  • Manufactured from PP, stable until -80 °C
  • Centrifuge at max. 25,000 x g with fixed angled rotors and 30,000 x g with swing out rotors
  • Maximal sample volume of 700 Μl
  • No cross contamination possible

 

further information on Chromatrap products (PDF)

Erweiterte Suche

Nutzen Sie die erweiterte Suche, um gezielt nach Produkten und Herstellern in unserem Sortiment zu filtern. Geben Sie einfach den gewünschten Hersteller ein oder verwenden Sie die verfügbaren Filter, um Ihre Auswahl einzugrenzen. So finden Sie schnell und präzise das passende Laborgerät für Ihren Bedarf.

Diese Site ist auf wpml.org als Entwicklungs-Site registriert. Wechseln Sie zu einer Produktionssite mit dem Schlüssel remove this banner.