Karriere im Labor und Vertrieb bei Dunn Labortechnik
Karriere im Labor und Vertrieb bei Dunn Labortechnik

Solid Phase Extraction (SPE)

Solid Phase Extraction (SPE)

Porvair offers a wide range of products for Solid Phase Extraction (SPE). The solved sample can be purified and concentrated to be eluted and  then analyzed by LC-MS.
  • Protein ‘crashes’ out of solution and precipitates directly in each well when acetonitrile is added, thus solving all common problems associated with the CRASH technique of protein clean-up.
  • Pre-filter frit at 100 Μm traps flocculant particles >100 Μm
  • Secondary frit traps fine protein particles at <10Μm
  • The frits are hydrophobic and oleophobic. This retains sample/acetonitrile in the well to allow precipitation of proteins until vacuum is applied.
  • Chemically inert filter material minimizes adsorption of samples.
  • Frit structure prevents breakthrough of protein particles.
  • PPLR frit consists of co-sintered plastics and chromatography media.
  • The hydrophobic top frit prevents sample passing into the media during mixing.
  • PPLR with 25 mg bed weight available as 1 ml cartridge or 96-well plate.
  further information about Microlute® products from Porvair (PDF)
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Dr. Kevin Denkmann

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