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Substrate

Substrate für die Immunologie und Molekularbiologie
(auch ultrasensitiv)

High Kinetic TMB

Kat.-Nr.	VE
Stabilzing Diluents These products help improve stability of Enzyme-Antibody/-Antigen conjugates in their ready-to-use form
Stabilizing Diluent for Alkaline Phosphatase Conjugates
ALPD 100 ALPD-500 ALPD-1000	100 ml 500 ml 1000 ml
Stabilizing Diluent for Horseradish Peroxidase Conjugates
HRPD-100 HRPD-500 HRPD-1000	100 ml 500 ml 1000 ml
Stable Liquid substrates for HRP
Soluble Substrates
TMB-HK (2.5 mMol/L)
One-component. Stable for 18 months at RT, or 24 months at 2 - 8 °C. TMBHK (High Kinetic TMB) achieves the highest Optical Density with colour development in as little as 1 minute! Incubate 2-15 minutes and stop with Hydrochloric or Sulfuric Acid to obtain a stable yellow colour. Read at 450nm. Applications include visual assays or assays that need high sensitivity in short incubation time.
TMBHK-100 TMBHK-500 TMBHK-1000	100 ml 500 ml 1000 ml
TMB-US (2.08 mMol/L)
One component. Stable for 18 months at RT, or 24 months at 2 - 8 °C. Ultra Sensitive TMB. Incubate 2-15 minutes and read the blue colour at 650 nm. Use of a stop solution produces a yellow end product that can be read at 450 nm. The yellow colour is stable for up to 2 hours!
TMBUS-100 TMBUS-500 TMBUS-1000	100 ml 500 ml 1000 ml
TMB-E (1.25 mMol/L)
One component. Stable for 18 months at RT, or 24 months at 2 - 8 °C. Suitable for most ELISA assays. Incubate 5-15 minutes and read the blue colour at 650 nm. For added sensitivity, adding a stop solution produces a yellow colour that can be read at 450 nm for up to 10-20 minutes after stopping.
TMBE-100 TMBE-500 TMBE-1000	100 ml 500 ml 1000 ml
TMB-D
Optimization Buffer for TMB-US. Used for diluting TMB to achieve desired OD reading while maintaining stability
TMBD-1000	1000 ml
TMB-Stable Stop (1.56 mMol/L)
One component. Stable for 18 months at RT, or 24 months at 2 - 8 °C. Same as TMB-US, but slower acting. Can be used for most ELISA assays for kinetic or endpoint reactions. The yellow product is stable for at least 1 hour displaying less than a 1 % loss in absorbance.
TMBE-100S TMBE-500S TMBE-1000S	100 ml 500 ml 1000 ml
ABTS (1.46 mMol/L)
One component. Stable for 18 months at RT, or 24 months at 2 - 8 °C. ABTS is a soluble, safe, convenient and good overall performing substrate for ELISA. ABTS develops an intense blue-green colour measurable between 405 nm and 410 nm. It may be used for both kinetic and endpoint reactions.
ABTS-100 ABTS-500 ABTS-1000	100 ml 500 ml 1000 ml
Insoluble Substrates
TMB-M (1.13 mMol/L)
One component. Stable for 18 months at RT, or 24 months at 2 - 8 °C. A peroxidase substrate which produces a stable, insoluble aquamarine blue precipitate at the reaction site with little or no background. It is ideal for immunoblotting procedures and can significantly increase the detection limits of assays on a variety or membranes.
TMBM-100 TMBM-500 TMBM-1000	100 ml 500 ml 1000 ml
TMB-H (1.25 mMol/L)
One component. Stable for 18 months at RT, or 24 months at 2 - 8 °C. Same as TMBM, but additionally may be used for immunohistochemical procedures and in situ hybridization techniques.
TMBH-100 TMBH-500 TMBH-1000	100 ml 500 ml 1000 ml
AEC (0.095 Mol/L) Two component system. Stable for 18 months at RT. A precipitable peroxidase substrate for immunoblotting and immunohistochemical staining techniques. AEC produces a red end product that is soluble in alcohol. When used in immunohistology, aqueous mounting media and counterstains must be used.
AECM-10 (50x) AECM-100 (50x) Buffer AECB-50 (10x) Buffer AECB-500 (10x)	10 ml 100 ml 50 ml 500 ml
DAB (0.0694 Mol/L) Two component system. Stable for 18 months at 2 - 8 °C. This substrate is widely used for both immunoblotting and immunohistochemical staining techniques. It produces an insoluble end product that is brown in colour and insoluble in alcohol. It is supplied as 50x concentrate. DAB is a suspected carcinogen. Buffer is supplied as 10x concentrate (pH 7.6 when diluted).
DABM-10 DABM-100 DABM-500 Buffer DABB-50 Buffer DABB-500 Buffer DABB-2500	10 ml 100 ml 500 ml 50 ml 500 ml 2500 ml
Stable Liquid Substrates for ALP
Soluble Substrates Improved Quality
p-NPP (4.33 mMol/L) New Formulation, more stable, lower background One component. Stable for 18 months at 2 - 8 °C. This is an excellent substrate for phosphatase based ELISA assays. It produces a soluble end product which is yellow in colour and reads at 405 nm to 410 nm. Both kinetic and end point determinations can be performed. This product is buffered in DEA.
NPPD-100 NPPD-500 NPPD-1000	100 ml 500 ml 1000 ml
p-NPP (50x) (0.19 Mol/L) One component. Stable for 18 months at 2 - 8 °C. This is an excellent substrate for phosphatase based ELISA assays. It produces a soluble end product which is yellow in colour and reads at 405 nm to 410 nm. Both kinetic and end point determinations can be performed. It is supplied as 50x concentrate. (Requires DEA buffer.)
PNPP-10 PNPP-100 PNPP-500 PNPP-1000	10 ml 100 ml 500 ml 1000 ml
DEA Buffer DEA buffer is ready to use. Use with p-NPP concentrate listed above.
PNPB-500 PNPB-5L PNPB-25L PNPB-50L	500 ml 5 liters 25 liters 50 liters
Insoluble Substrates
BCIP/NBT Plus (BCIP 0.692 mMol/L) (NBT 0.734 mMol/L)
One component. Stable for 18 months at RT, or 24 months at 2 - 8 °C. This reagent may be used for immunoblotting, immunohistochemical and in situ hybridization procedures. The system is based on hydrolysis of BCIP and reduction of NBT producing a deep purple reaction product. This single component substrate improves sensitivity and produces little or no background staining.
NBTH-100 NBTH-500 NBTH-1000	100 ml 500 ml 1000 ml
BCIP/NBT (BCIP 0.577 mMol/L) (NBT 0.122 mMol/L)
One component. Stable for 18 months at RT, or 24 months at 2 - 8 °C. Similar to the above product but formulated for immunoblotting procedures only.
NBTM-100 NBTM-500 NBTM-1000	100 ml 500 ml 1000 ml
BCIP (2.31 mMol/L)
One component. Stable for 18 months at 2 - 8 °C. When reacted with alkaline phosphatase, the free halogenated indoxyl derivative dimerizes forming an insoluble blue indigo dye. This product is useful for immunoblotting and immunohistochemical procedures.
BCIP-100 BCIP-500 BCIP-1000	100 ml 500 ml 1000 ml
NBT (0.489 mMol/L)
One component. Stable for 24 months at 2 - 8 °C. This buffered solution of NBT has been proven useful in analyte detection systems that employ dehydrogenase enzyme chemistry.
NBTS-100 NBTS-500 NBTS-1000	100 ml 500 ml 1000 ml
BCIP/TNBT (BCIP 0.452 mMol/L) TNBT 0.432 mMol/L)
One component. Stable for 18 months at RT, or 24 months at 2 - 8 °C. Similar to BCIP/NBT Plus but is more sensitive and produces a more intense purple colour. This is extremely useful in double antibody staining procedures when used with BCIP/INT where phosphatase labeled antibodies are preferred.
TNBT-100 TNBT-500 TNBT-1000	100 ml 500 ml 1000 ml
Fast Red Two component. Stable for 18 months at 2 - 8 °C. Used for tissue sections or immunoblotting. Uses LB salt which produces a brilliant red colour at reaction site. Easy to use. Simply mix the 2 liquid components 1:1 and apply.
ASLB-100 ASLB-500 ASLB-1000	100 ml 500 ml 1000 ml
Þ Reliable performance - all substrates are exceptionally stable in liquid form for 18-24 months, assuring extended kit shelf life.
Þ Non Toxic base matrix - easy to handle and dispose of. No preservatives. Increased consistency.
Þ Lot-to-lot consistency - ensured by maintaining sequestered raw materials and consistent manufacturing protocols.
Þ Moss Inc. can supply products in bulk or pre-dispensed format to your specifications.

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